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eclipse e200 fluorescence microscope  (Nikon)


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    Nikon eclipse e200 fluorescence microscope
    Eclipse E200 Fluorescence Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 4418 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/eclipse+e200+fluorescence+microscope/pm41863612-74-7-6?v=Nikon
    Average 99 stars, based on 4418 article reviews
    eclipse e200 fluorescence microscope - by Bioz Stars, 2026-07
    99/100 stars

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    A Evaluation of the internalization of the five monoclonal antibodies against the JAM-A protein. Treatments were carried out for 0 hours (30 minutes of incubation with each antibody at 37 º C followed by fixation) and 12 hours (treatment for 30 minutes, following by washing and incubation for 12 hours at 37 º C). White arrows indicate intracellular accumulations of the antibody signal. Inmunofluorescence was then performed as indicated in methods. Images were taken using a Nikon Eclipse <t>E200</t> inverted fluorescence microscope and processed using ImageJ software. Computed binding free energy plotted as a function of the experimentally measured percentage of antibody internalization. Each point represents an antibody variant. Shaded regions red and green indicate energetic internalization regimes incompatible or compatible respectively.
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    A Evaluation of the internalization of the five monoclonal antibodies against the JAM-A protein. Treatments were carried out for 0 hours (30 minutes of incubation with each antibody at 37 º C followed by fixation) and 12 hours (treatment for 30 minutes, following by washing and incubation for 12 hours at 37 º C). White arrows indicate intracellular accumulations of the antibody signal. Inmunofluorescence was then performed as indicated in methods. Images were taken using a Nikon Eclipse <t>E200</t> inverted fluorescence microscope and processed using ImageJ software. Computed binding free energy plotted as a function of the experimentally measured percentage of antibody internalization. Each point represents an antibody variant. Shaded regions red and green indicate energetic internalization regimes incompatible or compatible respectively.
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    A Evaluation of the internalization of the five monoclonal antibodies against the JAM-A protein. Treatments were carried out for 0 hours (30 minutes of incubation with each antibody at 37 º C followed by fixation) and 12 hours (treatment for 30 minutes, following by washing and incubation for 12 hours at 37 º C). White arrows indicate intracellular accumulations of the antibody signal. Inmunofluorescence was then performed as indicated in methods. Images were taken using a Nikon Eclipse E200 inverted fluorescence microscope and processed using ImageJ software. Computed binding free energy plotted as a function of the experimentally measured percentage of antibody internalization. Each point represents an antibody variant. Shaded regions red and green indicate energetic internalization regimes incompatible or compatible respectively.

    Journal: bioRxiv

    Article Title: Computational mapping of antibody-receptor energy landscapes to predict membrane internalization

    doi: 10.64898/2026.03.13.711720

    Figure Lengend Snippet: A Evaluation of the internalization of the five monoclonal antibodies against the JAM-A protein. Treatments were carried out for 0 hours (30 minutes of incubation with each antibody at 37 º C followed by fixation) and 12 hours (treatment for 30 minutes, following by washing and incubation for 12 hours at 37 º C). White arrows indicate intracellular accumulations of the antibody signal. Inmunofluorescence was then performed as indicated in methods. Images were taken using a Nikon Eclipse E200 inverted fluorescence microscope and processed using ImageJ software. Computed binding free energy plotted as a function of the experimentally measured percentage of antibody internalization. Each point represents an antibody variant. Shaded regions red and green indicate energetic internalization regimes incompatible or compatible respectively.

    Article Snippet: Fluorescence images of the cells were obtained using a Nikon Eclipse E200 inverted fluorescence microscope and analyzed using ImageJ.

    Techniques: Bioprocessing, Incubation, Fluorescence, Microscopy, Software, Binding Assay, Variant Assay